Novel Bacteriocinogenic Lactobacillus plantarum Strains and Their Differentiation by Sequence Analysis of 16S rDNA, 16S-23S and 23S-5S Intergenic Spacer Regions and Randomly Amplified Polymorphic DNA Analysis
نویسندگان
چکیده
Morteza Shojaei Moghadam1, Hooi Ling Foo1,2*, Thean Chor Leow2,3, Raha Abdul Rahim2,3 and Teck Chwen Loh4 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia Department of Animal Science, Faculty of Agriculture, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
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Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region.
A rapid and reliable PCR-based method for distinguishing closely related species within two groups of lactobacilli is described. Primers complementary to species-specific sequences in the 16S/23S rDNA spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 strains. The strains belong to two groups of closely related Lactobacillus species; one composed o...
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